IL-12RB1 Protein, Human, Recombinant (His)

IL-12RB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is P42701-1.

IL-12RB1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 64,500
200 μg	約5 days	￥ 114,000
500 μg	約5 days	￥ 241,000

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized human IL12RB1 at 20 μg/ml (100 μl/well) can bind human IL12B with a linear range of 2.56-64 ng/ml.
説明	IL-12RB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is P42701-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P42701-1
別名	interleukin 12 receptor, β1, IL12RB, IMD30, IL-12R-BETA1, interleukin 12 receptor, beta 1, CD212, IL-12R-β1
Construction	The Human IL12RB1 isoform 1 (NP_005526.1) extracellular domain (Met1-Glu540) was expressed fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 94 % as determined by SDS-PAGE
分子量	58.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin 12 receptor, beta 1 is also known as IL-12 receptor beta component, IL-12R subunit beta-1, and CD212 antigen (CD212). IL12RB1(CD212) is a subunit of the interleukin 12 receptor. IL12RB1(CD212) is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukin 12 (IL12) with a low affinity and is thought to be a part of the IL12 receptor complex. IL12RB1(CD212) forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of IL12RB1 and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. IL12RB1(CD212) Functions as an interleukin receptor that binds interleukin-12 with low affinity and is involved in IL12 transduction. It is associated with IL12RB2 it forms a functional, high-affinity receptor for IL12. IL12RB1(CD212) associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

参考文献

1. Cleary AM, et al. (2003) Impaired accumulation and function of memory CD4 T cells in human IL-12 receptor beta 1 deficiency. J Immunol. 170 (1): 597-603. 2. Suzuki Y, et al. (1997) Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. Gene. 200 (1-2): 149-56. 3. Yamamoto K, et al. (1997) Assignment of IL12RB1 and IL12RB2, interleukin-12 receptor beta 1 and beta 2 chains, to human chromosome 19 band p13.1 and chromosome 1 band p31.2, respectively, by in situ hybridization. Cytogenet. Cell Genet. 77 (3-4): 257-8.

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IL-12RB1 Protein, Human, Recombinant (His)

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