IL-17A Protein, Mouse, Recombinant

IL-17A Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.5 kDa and the accession number is Q62386.

IL-17A Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 52,500
100 μg	約5 days	￥ 102,500
200 μg	約5 days	￥ 182,000
500 μg	約5 days	￥ 385,500

バルクのお問い合わせ

生物学的情報	1. Measured by its binding ability in a functional ELISA. Immobilized mouse Il17A at 2 μg/mL (100 μl/well) can bind mouse IL17RA-His , The EC50 of mouse IL17RA-His is 12-45 ng/mL. 2. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50 for this effect is 0.15-0.6 ng/mL.
説明	IL-17A Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.5 kDa and the accession number is Q62386.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	Q62386
別名	IL-17A, Ctla8, Ctla-8, interleukin 17A, Il17, IL-17
Construction	A DNA sequence encoding the mature form of mouse IL17a (Q62386) (Thr22-Ala158) was expressed with a N-terminal Met.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	15.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7. 2. Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52. 3. Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52. 4. Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

IL-17A Protein, Mouse, Recombinant

参考文献

Calculator

技術サポート

Keywords