IL-17RD Protein, Human, Recombinant (His)

IL-17RD Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.5 kDa and the accession number is Q8NFM7-1.

IL-17RD Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000
1 mg	約5 days	￥ 897,500

バルクのお問い合わせ

説明	IL-17RD Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.5 kDa and the accession number is Q8NFM7-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q8NFM7-1
別名	IL-17RD, interleukin 17 receptor D, HH18, IL17RLM, SEF
Construction	extracellular domain of Human IL17RD (NP_060033.3) precursor (Met 1-Arg 299)
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	33.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-17 receptor D (IL-17D) also known as Interleukin-17 receptor-like protein, is a member of the interleukine-17 receptor family. IL-17RD functions as a feedback inhibitor of fibroblast growth factor-mediated Ras-MAPK signaling and ERK activation. It may inhibit FGF-induced FGFR1 tyrosine phosphorylation, regulate the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK By similarity, and mediate JNK activation and may be involved in apoptosis. IL-17RD is found expressed in the neopallial cortex, rhombic lip, and dorsal regions of the myelencephalon and the frontal nasal process. IL-17RD is also expressed in the commissural plate and septal area of the forebrain and the hippocampus, lens, and optic cup. In the oral region, IL-17RD is expressed in the tongue and the mesenchyme of the first branchial arch. It is also expressed in the developing inner ear. IL-17RD interacts with both IL-17R-Myc and IL-17RB-Myc. Both the intracellular and extracellular domains of IL-17RD interact with IL-17R. IL-17R forms a heteromeric complex with IL-17RD. Experiment results indicate that IL-17RD can affect IL-17R localization, suggesting that these two molecules are colocalized and associate with each other within cells. The fact that IL-17RD Delta ICD is unable to mediate IL-17 signaling but functions as a dominant-negative form indicates that the intracellular domain of IL-17RD is pivotal. Also, IL-17RD interacts with the IL-17R downstream molecule TRAF6. It has been proposed that the IL-17RD intracellular domain interacts with IL-17R and TRAF6 to deliver the downstream signal.

参考文献

1. Weaver CT,et al.. (2007) IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu Rev Immunol. 25: 821-52. 2. Rong Z,et al.. (2009) IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Cell Res. 19(2): 208-15. 3. Gaffen SL. (2009) Structure and signalling in the IL-17 receptor family. Nat Rev Immunol. 9(8): 556-67.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

IL-17RD Protein, Human, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords