IL-18BP Protein, Human, Recombinant (aa 1-192, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.4 kDa and the accession number is AAD17187.1.
生物学的情報 |
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.1‑0.6 µg/mL in the presence of 50 ng/mL of recombinant human IL-18.
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説明 | IL-18BP Protein, Human, Recombinant (aa 1-192, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 44.4 kDa and the accession number is AAD17187.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-hFc |
Accession Number | AAD17187.1 |
別名 | interleukin 18 binding protein, IL18BPa |
Construction | The Human IL18BP (AAD17187.1) (Met1-Gly192) was expressed with the Fc region of Human IgG1 at the C-terminus. |
Protein Purity |
> 95 % as determined by SDS-PAGE.
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分子量 | 44.4 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-18-binding protein (IL-18BP) is a constitutively expressed and secreted protein. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18) and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. The adjacently located family members IL18 Receptor 1 (IL18R1) and IL18 receptor accessory protein (IL18RAP) may also be important in the development of asthma and atopy. IL-18 binding protein (IL-18BP) was only moderately elevated, resulting in a high level of biologically active free IL-18 in HPS. A severe IL-18/IL-18BP imbalance results in Th-1 lymphocyte and macrophage activation, which escapes control by NK-cell cytotoxicity and may allow for secondary HPS in patients with underlying diseases. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-18BP Protein, Human, Recombinant (aa 1-192, hFc) interleukin 18 binding protein IL18BPa recombinant recombinant-proteins proteins protein