IL-21 Protein, Human, Recombinant

IL-21 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.6 kDa and the accession number is Q9HBE4-1.

IL-21 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 41,000
100 μg	約5 days	￥ 102,500
200 μg	約5 days	￥ 181,500
500 μg	約5 days	￥ 383,500

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生物学的情報	Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
説明	IL-21 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15.6 kDa and the accession number is Q9HBE4-1.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	Q9HBE4-1
別名	CVID11, IL-21, Za11, interleukin 21
Construction	The mature form of Human IL-21 (Q9HBE4-1) (Gln30-Ser162) was expressed with an initial Met.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	15.6 kDa (predicted)

Endotoxin	< 5 EU/mg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation).Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96. 2. Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10. 3. Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4. Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34.

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IL-21 Protein, Human, Recombinant

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