IL-25/IL17E Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 45 kDa and the accession number is Q9H293-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
50 μg | 在庫あり | ¥ 45,500 |
100 μg | 約5 days | ¥ 77,000 |
200 μg | 約5 days | ¥ 132,000 |
生物学的情報 |
1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His at 10 μg/ml (100 μl/well) can bind human Fc-IL25 , The EC50 of human Fc-IL25 is 1.7-4.2 μg/mL.
2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL.
|
説明 | IL-25/IL17E Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 45 kDa and the accession number is Q9H293-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | hFc |
Accession Number | Q9H293-1 |
別名 | interleukin 25, IL17E |
Construction | The mature form of Human IL25 (Tyr 33-Gly177) (NP_073626.1) was fused with the Fc region of Human IgG1 at the N-terminus via a polypeptide linker. |
Protein Purity |
> 95 % as determined by SDS-PAGE
|
分子量 | 45 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS , pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
IL-25/IL17E Protein, Human, Recombinant (hFc) interleukin 25 IL17E recombinant recombinant-proteins proteins protein