IL-2 Protein, Human, Recombinant (E. coli) is expressed in E. coli expression system. The accession number is P60568.
| 生物学的情報 |
1. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Human IL-2 with an affinity constant of 17.0 nM as determined in BLI assay. (Regularly tested)
2. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ≥1×10^7 IU/mg. (QC verified)
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| 説明 |
IL-2 Protein, Human, Recombinant (E. coli) is expressed in E. coli expression system. The accession number is P60568. |
| Species |
Human
|
| Expression Host |
E. coli
|
| Tag |
Tag Free
|
| Accession Number |
P60568
|
| 別名 |
Aldesleukin, IL-2, IL2, T-Cell Growth Factor, TCGF, Interleukin-2
|
| Amino Acid |
Ala21-Thr153
|
| Construction |
Ala21-Thr153
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| Endotoxin |
< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 10 mM sodium citrate, pH 4.0.
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| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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| Research Background |
Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system，belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.
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