IL-2 Protein, Mouse, Recombinant

IL-2 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.4kDa and the accession number is P04351.

IL-2 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 24,500
100 μg	約5 days	￥ 68,000

バルクのお問い合わせ

生物学的情報	1. Measured by its ability to bind recombinant human IL2Ra-Fc in a functional ELISA. 2. Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 6-30 ng/mL.
説明	IL-2 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.4kDa and the accession number is P04351.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P04351
別名	Il-2, interleukin 2
Construction	The soluble form of Mouse IL2 (P04351) (Ala 21-Gln 169) was expressed, with an initial Met at the C-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
分子量	17.4kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 2 mM DTT, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-2, also known as a T-cell growth factor, TCGF, Aldesleukin, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells).Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2 and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy is ineffective.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Smith KA, et al.,1980, J. Exp. Med.151 (6): 1551-6. 2. Smith KA, et al.,1980, Nature. 287 (5785): 853-5. 3. Taniguchi T, et al.,1983, Nature. 302 (5906): 305. 4. Cantrell DA, et al.,1984, Science. 224 (4655): 1312-6. 5. Smith KA, et al.,1988, Science. 240 (4856): 1169-76. 6. Wang X. et al., 2005, Science 310:1159-63. 7. Stauber D.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 2788-93.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

IL-2 Protein, Mouse, Recombinant

参考文献

Calculator

技術サポート

Keywords