IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158)

IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is Q9UHA7.

IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158)

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 45,500
100 μg	約5 days	￥ 156,500
200 μg	約5 days	￥ 266,500
500 μg	約5 days	￥ 539,500

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生物学的情報	Measured by its ability to induce IL8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is typically 5-20 ng/mL.
説明	IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is Q9UHA7.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	Q9UHA7
別名	interleukin 36, alpha, FIL1(ε), IL1(ε), IL1E, IL-1F6, FIL1E, IL1F6, IL1(EPSILON), FIL1, FIL1(EPSILON), IL36 α/IL-1F6, IL36A, FIL, 1E, interleukin 36, α
Construction	The Human IL1F6 (Q9UHA7) (Ala6-Phe158) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	17 .2 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine that plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

参考文献

1. Tripodi D,et al. (2012) IL-36 a new member of the IL-1 family cytokines. J Biol Regul Homeost Agents. 26(1):7-14. 2. Towne JE,et al. (2004) Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J Biol Chem. 279(14): 13677-88.

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IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158)

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