IL-36 alpha/IL-1F6 Protein, Mouse, Recombinant

IL-36 alpha/IL-1F6 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is Q9JLA2-1.

IL-36 alpha/IL-1F6 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 106,000

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説明	IL-36 alpha/IL-1F6 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is Q9JLA2-1.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	Q9JLA2-1
別名	IL1(ε), FIL1(ε), IL36 α/IL-1F6, IL1F6, IL1(EPSILON), FIL1, interleukin 36, alpha, FIL1E, interleukin 36, α, FIL1(EPSILON), IL-1F6
Construction	The Mouse IL1F6 (Q9JLA2-1) (Met 1-His 160) was expressed and purified.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	18 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine that plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

参考文献

1. Tripodi D,et al. (2012) IL-36 a new member of the IL-1 family cytokines. J Biol Regul Homeost Agents. 26(1):7-14. 2. Towne JE,et al. (2004) Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J Biol Chem. 279(14): 13677-88. 3. Kumar S,et al. (2000) Identification and initial characterization of four novel members of the interleukin-1 family. J Biol Chem. 275(14): 10308-14.

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IL-36 alpha/IL-1F6 Protein, Mouse, Recombinant

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