IL-36RN/IL-1F5 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17 kDa and the accession number is Q9QYY1.
説明 | IL-36RN/IL-1F5 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17 kDa and the accession number is Q9QYY1. |
Species | Mouse |
Expression Host | E. coli |
Tag | Tag Free |
Accession Number | Q9QYY1 |
別名 | Fil1δ, AI413231, RP23-176J12.6, Fil1delta, Il1hy1, Il-1h3, interleukin 36 receptor antagonist |
Construction | The Mouse IL1F5 (NP_062324.2) (Val 3-Asp 156) was expressed and purified.. |
Protein Purity | > 94 % as determined by SDS-PAGE |
分子量 | 17 kDa (predicted) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist, and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in the local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR-/- mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain. |
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IL-36RN/IL-1F5 Protein, Mouse, Recombinant Fil1δ AI413231 RP23-176J12.6 Fil1delta AI-413231 Il1hy1 Il-1h3 AI 413231 interleukin 36 receptor antagonist recombinant recombinant-proteins proteins protein