IL-10R alpha/IL-10RA Protein, Cynomolgus, Recombinant (His)

IL-10R alpha/IL-10RA Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25.34 kDa and the accession number is A0A2K5WJ66.

IL-10R alpha/IL-10RA Protein, Cynomolgus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明	IL-10R alpha/IL-10RA Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25.34 kDa and the accession number is A0A2K5WJ66.

Species	Cynomolgus
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	A0A2K5WJ66
別名	HIL-10R, IL-10R subunit 1, IL10RA, IL-10 R alpha, IL-10R1, CDw210a, IL-10 R α, IL-10RA, IBD28, IL10R
Construction	His22-Asn233
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	25.34 kDa (predicted). Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

参考文献

1. Mallikarjunappa S, et al. Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9. BMC Genet. 2020 Nov 2;21(1):12doi: 10.1186/s12863-020-00925-4. PMID: 33138773; PMCID: PMC7607837.

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IL-10R alpha/IL-10RA Protein, Cynomolgus, Recombinant (His)

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