IL-13 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 13-30 KDa and the accession number is AAH96139.
| 生物学的情報 |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1.5-4.5 ng/ml. (QC verified)
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| 説明 |
IL-13 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 13-30 KDa and the accession number is AAH96139. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-6xHis
|
| Accession Number |
AAH96139
|
| 別名 |
Interleukin-13, IL-13
|
| Amino Acid |
Gly35-Asn146
|
| Construction |
Gly35-Asn146
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
13-30 KDa (reducing condition)
|
| Endotoxin |
< 10 EU/µg as determined by LAL test.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
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| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
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| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
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| Research Background |
Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.
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