IL-13 Protein, Mouse, Recombinant (aa 26-131, His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 15-30 KDa and the accession number is P20109.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
10 μg | 在庫あり | ¥ 27,000 |
50 μg | 約5 days | ¥ 80,500 |
500 μg | 約5 days | ¥ 542,000 |
1 mg | 約5 days | ¥ 870,000 |
生物学的情報 |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml. (QC verified)
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説明 | IL-13 Protein, Mouse, Recombinant (aa 26-131, His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 15-30 KDa and the accession number is P20109. |
Species | Mouse |
Expression Host | Human Cells |
Tag | C-6His |
Accession Number | P20109 |
別名 | Interleukin-13, T-Cell Activation Protein P600, IL-13, Il13 |
Amino Acid | Ser26-Phe131 |
Construction | Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Ser26-Phe131 is expressed with a 6His tag at the C-terminus. |
Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
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分子量 | 15-30 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-13 Protein, Mouse, Recombinant (aa 26-131, His) Interleukin-13 T-Cell Activation Protein P600 T-Cell Activation Protein P 600 IL-13 Il13 T-Cell Activation Protein P-600 IL 13 recombinant recombinant-proteins proteins protein