IL-18 Protein, Rhesus, Recombinant (His)

IL-18 Protein, Rhesus, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 23.7 kDa and the accession number is Q9BGI5.

IL-18 Protein, Rhesus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
5 μg	在庫あり	￥ 13,000
10 μg	約5 days	￥ 20,500
20 μg	約5 days	￥ 31,500
50 μg	約5 days	￥ 56,500
100 μg	約5 days	￥ 96,000
200 μg	約5 days	￥ 163,500
500 μg	約5 days	￥ 328,500

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生物学的情報	Testing in progress
説明	IL-18 Protein, Rhesus, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 23.7 kDa and the accession number is Q9BGI5.

Species	Rhesus
Expression Host	E. coli
Tag	N-His
Accession Number	Q9BGI5
別名	interleukin 18
Construction	The rhesus IL-18 (NP_001028006.1) (Ala 2-Asp 193) was expressed with an N-terminal polyhistidine tag.
Protein Purity	> 92 % as determined by SDS-PAGE
分子量	23.7 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24. 2. Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90. 3. Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.

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IL-18 Protein, Rhesus, Recombinant (His)

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