IL-18R alpha Protein, Mouse, Recombinant (mFc)

IL-18R alpha Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 61.74 kDa and the accession number is Q61098.

IL-18R alpha Protein, Mouse, Recombinant (mFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 58,500
100 μg	約5 days	￥ 100,000
200 μg	約5 days	￥ 171,000
500 μg	約5 days	￥ 346,500

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized mIL18R1-mFc(mIgG2a) at 10 μg/mL (100 μL/well) can bind mIL18/Biotin , the EC50 of mIL18/Biotin is 90-260 ng/mL.
説明	IL-18R alpha Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 61.74 kDa and the accession number is Q61098.

Species	Mouse
Expression Host	HEK293
Tag	mFc
Accession Number	Q61098
別名	Il18rα, Il1rrp, interleukin 18 receptor 1, Il18ralpha
Construction	A DNA sequence encoding the mouse IL18R1 (NP_032391.1) (Met1-Gly326) was expressed with the Fc region of mouse IgG2a at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	61.74 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin-18 receptor 1 (IL18R1) also known as CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, and CD218a, is an interleukin receptor of the immunoglobulin superfamily. IL18R1 is found expressed in the lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from the brain, skeletal muscle, pancreas, and kidney. A high level of expression is found in Hodgkin disease cell lines. This receptor is specifically bound to interleukin 18 (IL18) and is essential for IL18 mediated signal transduction. IL18R1 contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. It is a single-pass type I membrane protein. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The increased expression of IL18R1 may contribute pathogenically to disease and is therefore a potential therapeutic target. The absence of a genetic association in the IL18R1 gene itself suggests regulation from other parts of the genome, or as part of the inflammatory cascade in multiple sclerosis without a prime genetic cause.

参考文献

1. Nadif R,et al.. (2006) IL18 and IL18R1 polymorphisms, lung CT and fibrosis: A longitudinal study in coal miners. Eur Respir J. 28(6): 1100-5. 2. Haralambieva IH,et al.. (2011) Common SNPs/haplotypes in IL18R1 and IL18 genes are associated with variations in hum oral immunity to smallpox vaccination in Caucasians and African Americans. J Infect Dis. 204(3): 433-41. 3. Hulin-Curtis SL,et al.. (2012) Evaluation of IL18 and IL18R1 polymorphisms: genetic susceptibility to knee osteoarthritis. Int J Immunogenet. 39(2): 106-9.

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IL-18R alpha Protein, Mouse, Recombinant (mFc)

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