IL-1 alpha/IL-1A Protein, Human, Recombinant

IL-1 alpha/IL-1A Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18 kDa and the accession number is P01583.

IL-1 alpha/IL-1A Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 41,000
100 μg	約5 days	￥ 102,500
200 μg	約5 days	￥ 182,000
500 μg	約5 days	￥ 385,500

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生物学的情報	Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.07-0.35 ug/mL.
説明	IL-1 alpha/IL-1A Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18 kDa and the accession number is P01583.

Species	Human
Expression Host	HEK293
Tag	Tag Free
Accession Number	P01583
別名	IL-1F1, interleukin 1, α, interleukin 1, alpha, IL1-ALPHA, IL-1 α/IL1A, IL1, IL-1A, IL1-α, IL1F1
Construction	The processed form of human IL1α (NP_000566.3) (Ser 113-Ala 271) was expressed and purified.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	18 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Nicklin MJ,et al.(1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19(2):382-4. 2. March CJ,et al.(1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 315(6021):641-7. 3. Bankers-Fulbright JL,et al.(1996) Interleukin-1 signal transduction. Life Sci. 59(2):61-83. 4. Dinarello CA,et al.(1997) Induction of interleukin-1 and interleukin-1 receptor antagonist. Semin Oncol. 24 (3 Suppl 9):S9-81-S9-93.

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IL-1 alpha/IL-1A Protein, Human, Recombinant

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