IL-22 Protein, Human, Recombinant (hIgG4 hFc) is expressed in HEK293 mammalian cells with C-hIgG4 Fc tag. The predicted molecular weight is 50-75 KDa and the accession number is Q9GZX6.
| 説明 |
IL-22 Protein, Human, Recombinant (hIgG4 hFc) is expressed in HEK293 mammalian cells with C-hIgG4 Fc tag. The predicted molecular weight is 50-75 KDa and the accession number is Q9GZX6. |
| Species |
Human
|
| Expression Host |
Human Cells
|
| Tag |
C-hIgG4 Fc
|
| Accession Number |
Q9GZX6
|
| 別名 |
IL-10-related T-cell-derived-inducible factor, Interleukin-22, IL22, IL-22, Cytokine Zcyto18, IL-TIF
|
| Amino Acid |
Ala34-Ile179
|
| Construction |
Recombinant Human Interleukin-22 is produced by our Mammalian expression system and the target gene encoding Ala34-Ile179 is expressed with a human IgG4 Fc tag at the C-terminus.
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
50-75 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
|
| Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|
| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
|
| Shipping |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
|
| Research Background |
Interleukin-22(IL-22) is a member of a group of the IL-10 family, a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells. IL-22 and IL-10 receptor chains play a role in cellular targeting and signal transduction. It can initiate and regulate innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 likely plays a role in the coordinated response of both adaptive and innate immune systems. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. Biological activity of IL-22 is initiated by binding to a cell-surface complex consisting of IL-22R1 and IL-10R2 receptor chains. IL-22 biological activity is further regulated by interactions with a soluble binding protein, IL-22BP. IL-22BP and an extracellular region of IL-22R1 share sequence similarity. In some cases, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by cytokine IL-17A.
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