IL-2RB & IL-2RG Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 54.4 kDa and the accession number is P14784-1.
| 説明 |
IL-2RB & IL-2RG Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 54.4 kDa and the accession number is P14784-1. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-Flag, C-His
|
| Accession Number |
P14784-1
|
| Construction |
The Human IL2RB (P14784-1)(Met1-Asp239) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The Human IL2RG (P31785-1) (Met1-Asn254) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL2RB & IL2RG heterodimer was purified.
|
| Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
| 分子量 |
54.4 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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