IL-33 Protein, Human, Recombinant

IL-33 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is O95760-1.

IL-33 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
5 μg	在庫あり	￥ 13,000
10 μg	約5 days	￥ 21,000
20 μg	約5 days	￥ 34,000
50 μg	約5 days	￥ 63,500
100 μg	約5 days	￥ 102,500
200 μg	約5 days	￥ 175,500
500 μg	約5 days	￥ 353,500

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生物学的情報	1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 1-7 ng/mL.
説明	IL-33 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is O95760-1.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	O95760-1
別名	IL1F11, C9orf26, NFEHEV, NF-HEV, DVS27, interleukin 33
Construction	The C-terminal segment of Human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	18 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

参考文献

1. Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8. 2. Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.

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IL-33 Protein, Human, Recombinant

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