IL-34 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 26.7 kDa and the accession number is AAH29804.1.
| 生物学的情報 |
Measured in a cell proliferation assay using human peripheral blood mononuclear cell. The ED50 for this effect is 2-8 ng/mL.
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| 説明 |
IL-34 Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 26.7 kDa and the accession number is AAH29804.1. |
| Species |
Human
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| Expression Host |
CHO
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| Tag |
His
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| Accession Number |
AAH29804.1
|
| 別名 |
IL-34, C16orf77, interleukin 34
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| Construction |
A DNA sequence encoding the human IL34 (AAH29804.1) (Met1-Pro242) was expressed with a polyhistidine tag at the C-terminus.
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| Protein Purity |
> 95 % as determined by SDS-PAGE.
|
| 分子量 |
26.7 kDa (predicted)
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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| Research Background |
IL34 (Interleukin 34) is a Protein Coding gene. IL-34, also known as uncharacterized protein C16 or f77 homolog, belongs to the IL-34 family. IL-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R). IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and most abundant in the spleen. The colony-stimulating factor 1 receptor (CSF-1R) is identified as the receptor for IL-34. IL-34 increases the growth or survival of immune cells known as monocytes. Besides, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
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参考文献
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