IL-35 Protein, Human, Recombinant (Flag & His)

IL-35 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 48.3 kDa and the accession number is P29459-1.

IL-35 Protein, Human, Recombinant (Flag & His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 79,500
200 μg	約5 days	￥ 150,500
500 μg	約5 days	￥ 349,000

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生物学的情報	Testing in progress
説明	IL-35 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 48.3 kDa and the accession number is P29459-1.

Species	Human
Expression Host	HEK293 Cells
Tag	Flag, His
Accession Number	P29459-1
別名	NKSF1, P35, Interleukin-35, CLMF, NFSK, IL-12A, IL-35, interleukin 12A
Construction	The Human IL27B (NP_005746.2)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The Human IL12A (P29459-1)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL27B&IL12A heterodimer was purified.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	48.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.

参考文献

1. Wolf S.F.,et al.,(1991), Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol. 146:3074-3081. 2. Gubler U.,et al., (1991), Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147. 3. Batten M.,et al.,(2007), The biology and therapeutic potential of interleukin 27.J. Mol. Med. 85:661-672.

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IL-35 Protein, Human, Recombinant (Flag & His)

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