IL-4 Protein, Mouse, Recombinant

IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.

IL-4 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
5 μg	在庫あり	￥ 12,000
10 μg	約5 days	￥ 19,000
20 μg	約5 days	￥ 30,500
50 μg	約5 days	￥ 57,500
100 μg	約5 days	￥ 93,000
200 μg	約5 days	￥ 165,000
500 μg	約5 days	￥ 544,000

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生物学的情報	Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-4 ng/mL.
説明	IL-4 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 13.4 kDa and the accession number is P07750.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P07750
別名	BSF-1, Il-4, interleukin 4
Construction	The Mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	13.4 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9. 2. Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3. 3. Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.

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IL-4 Protein, Mouse, Recombinant

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