IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99) is expressed in HEK293 mammalian cells. The predicted molecular weight is 8 kDa and the accession number is A0A024RDA5.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
10 μg | 在庫あり | ¥ 24,500 |
20 μg | 約5 days | ¥ 35,500 |
50 μg | 約5 days | ¥ 55,500 |
100 μg | 約5 days | ¥ 79,500 |
200 μg | 約5 days | ¥ 141,000 |
500 μg | 約5 days | ¥ 463,500 |
生物学的情報 | Testing in progress |
説明 | IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99) is expressed in HEK293 mammalian cells. The predicted molecular weight is 8 kDa and the accession number is A0A024RDA5. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | Tag Free |
Accession Number | A0A024RDA5 |
別名 | MONAP, MDNCF, NAP-1, NAP1, LUCT, chemokine (C-X-C motif) ligand 8, IL8, LYNAP, GCP1, NAF, GCP-1, LECT, IL-8, Interleukin-8 |
Construction | The 77 amino acid endothelial-cell derived form (Ala 23-Ser 99) of the mature Human IL8 (NP_000575.1) was expressed and purified. |
Protein Purity |
> 95 % as determined by SDS-PAGE
|
分子量 | 8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99) MONAP MDNCF NAP-1 NAP1 LUCT chemokine (C-X-C motif) ligand 8 IL8 LYNAP GCP 1 GCP1 NAP 1 NAF IL 8 GCP-1 LECT IL-8 Interleukin-8 recombinant recombinant-proteins proteins protein