IL-8/CXCL8 Protein, Human, Recombinant (His) is expressed in yeast with His tag. The predicted molecular weight is 9.8 kDa and the accession number is A0A024RDA5.
パッケージサイズ | 在庫状況 | 単価(税別) |
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100 μg | 在庫あり | ¥ 79,500 |
200 μg | 約5 days | ¥ 134,500 |
500 μg | 約5 days | ¥ 266,500 |
生物学的情報 |
Immobilized Human IL-8/CXCL8 (His Tag) at 2 μg/mL (100 μL/well) can bind Anti-IL-8/CXCL8 Antibody, Mouse Monoclonal, the EC50 is 25-140 ng/mL.
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説明 | IL-8/CXCL8 Protein, Human, Recombinant (His) is expressed in yeast with His tag. The predicted molecular weight is 9.8 kDa and the accession number is A0A024RDA5. |
Species | Human |
Expression Host | P. pastoris (Yeast) |
Tag | C-His |
Accession Number | A0A024RDA5 |
別名 | Interleukin-8, GCP1, MONAP, LECT, LYNAP, NAF, GCP-1, LUCT, MDNCF, IL8, NAP-1, chemokine (C-X-C motif) ligand 8, IL-8, NAP1 |
Construction | The Human CXCL8 (NP_000575.1) (Ser28-Ser99) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 95 % as determined by SDS-PAGE.
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分子量 | 9.8 kDa (predicted) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-8/CXCL8 Protein, Human, Recombinant (His) Interleukin-8 GCP1 IL 8 MONAP LECT LYNAP NAF NAP 1 GCP-1 LUCT MDNCF IL8 NAP-1 chemokine (C-X-C motif) ligand 8 IL-8 GCP 1 NAP1 recombinant recombinant-proteins proteins protein