IL-T4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 51.06 kDa and the accession number is AAH36827.1.
説明 | IL-T4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 51.06 kDa and the accession number is AAH36827.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His, Avi |
Accession Number | AAH36827.1 |
別名 | ILT-4, MIR10, LIR-2, leukocyte immunoglobulin like receptor B2, CD85D, LIR2, ILT4, MIR-10 |
Construction | The Human LILRB2 (AAH36827.1) (Met1-Val461) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
分子量 | 51.06 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 0.02% Tween 80, 10% Glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | ILT4, also known as LILRB2, is a member of the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). ILT4 gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. Multiple transcript variants encoding different isoforms have been found for the ILT4 gene. ILT4 is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-T4 Protein, Human, Recombinant (His & Avi), Biotinylated ILT-4 MIR 10 MIR10 LIR 2 LIR-2 leukocyte immunoglobulin like receptor B2 CD85D LIR2 ILT4 ILT 4 MIR-10 recombinant recombinant-proteins proteins protein