Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is Q8BAC3.1.
| 説明 |
Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is Q8BAC3.1. |
| Species |
H1N1
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| Expression Host |
E. coli
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| Tag |
N-His
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| Accession Number |
Q8BAC3.1
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| 別名 |
cholinergic receptor, muscarinic 1
|
| Construction |
The influenza A virus (A/Brevig Mission/1/ 1918(H1N1)) matrix protein 1(Q8BAC3.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag.
|
| Protein Purity |
> 90 % as determined by SDS-PAGE
|
| 分子量 |
30 kDa (predicted)
|
| Endotoxin |
Please contact us for more information.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 1 mM EDTA, 50 mM Tris, 50 mM NaCl, 5% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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| Research Background |
The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.
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