Influenza A H3N2 (A/swine/Thailand/CU-P53/2012) Hemagglutinin/HA Protein (His)

Influenza A H3N2 (A/swine/Thailand/CU-P53/2012) Hemagglutinin/HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.9 kDa and the accession number is S4TZ09.

Influenza A H3N2 (A/swine/Thailand/CU-P53/2012) Hemagglutinin/HA Protein (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	Influenza A H3N2 (A/swine/Thailand/CU-P53/2012) Hemagglutinin/HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.9 kDa and the accession number is S4TZ09.

Species	H3N2
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	S4TZ09
別名	Harvey rat sarcoma viral oncogene homolog
Construction	The Influenza A virus (A/swine/Thailand/CU-P53/2012 (H3N2)) hemagglutinin (AGG53108) (Met1-Ser525), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	57.9 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考文献

1. White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005. 2. Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

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Influenza A H3N2 (A/swine/Thailand/CU-P53/2012) Hemagglutinin/HA Protein (His)

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