Jagged 1 Protein, Human, Recombinant (His)

Jagged 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 112 kDa and the accession number is P78504-1.

Jagged 1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 68,000
100 μg	約5 days	￥ 114,000
200 μg	約5 days	￥ 190,000
500 μg	約5 days	￥ 374,000

バルクのお問い合わせ

生物学的情報	Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 4-20 μg/mL.
説明	Jagged 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 112 kDa and the accession number is P78504-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P78504-1
別名	CD339, AHD, HJ1, AGS, AWS, JAGL1, Jagged 1
Construction	The Human JAG1 (NP_000205.1) extracellular domain (Met 1-Ser 1046) was expressed, fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	112 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Protein Jagged 1, also known as JAG1, JAGL1, and CD339, is a single-pass type I membrane protein that contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. JAG1/Jagged 1 seems to be involved in the early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly that consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left), and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.

参考文献

1. Oda T.et al., 1997, Nat. Genet. 16:235-242. 2. Krantz I.D. et al., 1998, Am. J. Hum. Genet. 62:1361-1369. 3. Li L. et al., 1998, Immunity. 8:43-55. 4. Jones E.A. et al., 2000, J. Med. Genet. 37: 658-662. 5. Roepke A.et al., 2003, Hum. Mutat. 21:100-100. 6. Jurkiewicz D.et al., 2005, Hum. Mutat. 25:321-321. 7. Warthen D.M.et al., 2006, Hum. Mutat. 27:436-443.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

Jagged 1 Protein, Human, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords