KEAP1 Protein, Human, Recombinant (His & GST)

KEAP1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 97.37 kDa and the accession number is A0A024R7C0.

KEAP1 Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 151,500
500 μg	約5 days	￥ 307,500

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生物学的情報	Testing in progress
説明	KEAP1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 97.37 kDa and the accession number is A0A024R7C0.

Species	Human
Expression Host	Baculovirus-Insect Cells
Tag	His,GST
Accession Number	A0A024R7C0
別名	kelch like ECH associated protein 1, INRF2, KEAP-1, KLHL19
Construction	A DNA sequence encoding the human KEAP1 (NP_036421.2) (Gln2-Cys624) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	97.37 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Kelch-like ECH-associated protein 1, also known as a cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1, and INRF2, is a cytoplasm and nucleus protein that contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain, and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with the highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.

参考文献

1. Strachan GD. et al., 2004, Biochemistry. 43: 12113-22. 2. Zhang DD. et al., 2004, Mol Cell Biol. 24: 10941-53. 3. Zhang DD. et al., 2003, Mol Cell Biol. 23: 8137-51. 4. Lo S-C. et al., 2006, J Biol Chem. 281: 37893-903. 5. Buckley BJ. et al., 2008, Free Radic Biol Med. 44 (4) :692-8.

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KEAP1 Protein, Human, Recombinant (His & GST)

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