KLKB1 Protein, Mouse, Recombinant (His)

Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. KLKB1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 70.3 kDa and the accession number is P26262.

KLKB1 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 107,000
500 μg	約5 days	￥ 424,500
1 mg	約5 days	￥ 711,500

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説明

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P26262
別名	Kininogenin, KLK3, Fletcher factor, Plasma kallikrein, KLKB1, PKK
Construction	Gly20-Ala638
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	70.3 kDa (predicted). Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

参考文献

1. Abrera-Abeleda MA, et al. Mesangial immune complex glomerulonephritis due to complement factor D deficiency. Kidney Int. 2007 Jun;71(11):1142-7. doi: 10.1038/sj.ki.5002235. Epub 2007 Apr 4. PMID: 17410102.

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KLKB1 Protein, Mouse, Recombinant (His)

参考文献

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