LDLR Protein, Human, Recombinant (His)

LDLR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 86 kDa and the accession number is A0A024R7D5.

LDLR Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 48,000
100 μg	約5 days	￥ 82,500
200 μg	約5 days	￥ 140,000
500 μg	約5 days	￥ 438,500

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生物学的情報	Immobilized Human LDLR/LDL Receptor His at 2 μg/mL (100 μL/well) can bind Mouse PCSK9 mFc , the EC50 of Mouse PCSK9 mFc is 100-800 ng/mL.
説明	LDLR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 86 kDa and the accession number is A0A024R7D5.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	A0A024R7D5
別名	low density lipoprotein receptor, FHC, FH, LDLCQ2, LDL Receptor, LDL R
Construction	extracellular domain of Human LDLR (NP_000518.1) precursor (Met 1-Arg 788)
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	86 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

参考文献

1. Yamamoto T,et al.(1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38. 2. Pinson KI,et al.(2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.

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LDLR Protein, Human, Recombinant (His)

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