LIF Protein, Human, Recombinant

LIF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.7 kDa and the accession number is P15018-1.

LIF Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 36,000
100 μg	約5 days	￥ 91,500

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生物学的情報	Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-0.8 ng/mL.
説明	LIF Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.7 kDa and the accession number is P15018-1.

Species	Human
Expression Host	HEK293
Tag	Tag Free
Accession Number	P15018-1
別名	CDF, DIA, MLPLI, Leukemia Inhibitory Factor, HILDA
Construction	A DNA sequence encoding the human LIF (P15018-1) (Met1-Phe202) was expressed.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	19.7 kDa (predicted)

Endotoxin	< 10 EU per mg of the protein.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.

参考文献

1. Salas EM,et al. (2011) LIF, a Novel STAT5-Regulated Gene, Is Aberrantly Expressed in Myeloproliferative Neoplasms. Genes Cancer. 2 (5): 593-6. 2. Chodorowska G,et al. (2004) Leukemia inhibitory factor (LIF) and its biological activity. Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93. 3. Garcia-Campana AM,et al. (2007) LIF detection of peptides and proteins in CE. Electrophoresis. 28 (1-2): 208-32.

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LIF Protein, Human, Recombinant

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