LIF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
パッケージサイズ | 在庫状況 | 単価(税別) |
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20 μg | 在庫あり | ¥ 24,500 |
100 μg | 約5 days | ¥ 114,500 |
200 μg | 約5 days | ¥ 189,000 |
500 μg | 約5 days | ¥ 569,500 |
生物学的情報 |
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
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説明 | LIF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19.9 kDa and the accession number is P09056. |
Species | Mouse |
Expression Host | HEK293 |
Tag | Tag Free |
Accession Number | P09056 |
別名 | leukemia inhibitory factor |
Construction | A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified. |
Protein Purity |
> 95 % as determined by SDS-PAGE
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分子量 | 19.9 kDa (predicted) |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins. |
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Please read the User Guide of Recombinant Proteins for more specific information.
LIF Protein, Mouse, Recombinant leukemia inhibitory factor recombinant recombinant-proteins proteins protein