LILRB3/CD85a Protein, Human, Recombinant (His)

LILRB3/CD85a Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.6 kDa and the accession number is AAI04994.1.

LILRB3/CD85a Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	LILRB3/CD85a Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.6 kDa and the accession number is AAI04994.1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	AAI04994.1
別名	LIR3, PIRB, CD85A, HL9, MGC138403, LIR-3, ILT-5, leukocyte immunoglobulin like receptor B3, XXbac-BCX105G6.7, LILRA6, ILT5
Construction	The Human LILRB3 (AAI04994.1) extracellular domain (Met 1-Glu 443) was expressed, with a polyhistidine tag at the C-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	47.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein that belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3 / CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3 / CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in the modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3 / CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found.

参考文献

1. Huang,J. et al., 2010, J Virol. 84 (18): 9463-71. 2. Arm J.P. et al., 1997, J. Immunol. 159:2342-2349. 3. Wende H. et al., 2000, Immunogenetics 51:703-713. 4. Yu L.-R. et al., 2007,J. Proteome Res. 6:4150-4162.

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LILRB3/CD85a Protein, Human, Recombinant (His)

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