LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Domain 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 14.26 kDa and the accession number is AAH26309.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 187,000 |
500 μg | 約5 days | ¥ 746,000 |
1 mg | 約5 days | ¥ 1,248,500 |
説明 | LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Domain 1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 14.26 kDa and the accession number is AAH26309.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | AAH26309.1 |
別名 | LILRB4, ILT-3, HM18, LIR5, CD85K, ILT3 |
Construction | Gln22-Thr118 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 14.26 kDa (predicted). Due to glycosylation, the protein migrates to 14.5-20 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. |
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Please read the User Guide of Recombinant Proteins for more specific information.
LILRB4/CD85k/ILT3 Domain 1 Protein, Human, Recombinant (His & Avi) LILRB4 LILRB-4 LIR-5 LILRB 4 ILT-3 LIR 5 HM18 HM-18 HM 18 LIR5 CD85K ILT 3 ILT3 recombinant recombinant-proteins proteins protein