LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc)

LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 74.4 kDa and the accession number is A0A024RDG6.

LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

バルクのお問い合わせ

説明	LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 74.4 kDa and the accession number is A0A024RDG6.

Species	Human
Expression Host	HEK293 Cells
Tag	His, hFc
Accession Number	A0A024RDG6
別名	LGP85, SR-BII, AMRF, EPM4, LIMPII, LIMP-2, HLGP85, CD36L2, scavenger receptor class B, member 2
Construction	The Human SCARB2 (NP_005497.1) extracellular domain (Arg 27-Thr 432) was fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	74.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type I II multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, and may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.

参考文献

1. Crombie, R. et al., 1998, J. Biol. Chem. 273: 4855-4863. 2. Febbraio, M. et al., 2001, J. Clin. Invest. 108: 785-791. 3. Kuronita, T. et al., 2002, J. Cell Sci. 115: 4117-4131. 4. Gamp, A.C. et al., 2003, Human Molecular Genetics. 12: 631-646. 5. Eskelinen, E.L. et al., 2003, Trends in Cell Biology. 13: 137-145. 6. Mulcahy, J.V. et al.,2004, Biochem. J. 377 (Pt 3): 741–747. 7. Yamayoshi, S. et al., 2009, Nat Med. 15 (7): 798-801.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc)

参考文献

Calculator

技術サポート

Keywords