LRP-6 Protein, Human, Recombinant (mFc)

Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.

LRP-6 Protein, Human, Recombinant (mFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 85,500
500 μg	約5 days	￥ 342,000
1 mg	約5 days	￥ 569,500

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説明

Species	Human
Expression Host	HEK293
Tag	C-mFc
Accession Number	O75581-1
別名	DCAD2, LRP-6, FLJ90421, LRP6, FLJ90062
Construction	Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Ala20-Pro630.[Accession \|O75581-1]
Protein Purity	> 90% as determined by Tris-Bis PAGE
分子量	The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.

参考文献

1. Ren S, et al. LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1440-5. doi: 10.1073/pnas.1211179110. Epub 2013 Jan 9. Erratum in: Proc Natl Acad Sci U S A. 2016 Nov 14;: PMID: 23302695; PMCID: PMC3557093.

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LRP-6 Protein, Human, Recombinant (mFc)

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