M-CSF/CSF1 Protein, Human, Recombinant

M-CSF/CSF1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.4 kDa and the accession number is P09603-2.

M-CSF/CSF1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 63,500
100 μg	約5 days	￥ 177,500
1 mg	約5 days	￥ 1,154,500

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説明	M-CSF/CSF1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.4 kDa and the accession number is P09603-2.

Species	Human
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	P09603-2
別名	CSF-1, MCSF, M-CSF, colony stimulating factor 1 (macrophage)
Construction	The Human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	18.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

参考文献

1. Pandit J. et al., 1992, Science. 258: 1358-62. 2. Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8. 3. Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11. 4. Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92. 5. Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.

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M-CSF/CSF1 Protein, Human, Recombinant

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