MAD2L1 Protein, Human, Recombinant (His)

カタログ番号 TMPY-02238

別名: MAD2 mitotic arrest deficient-like 1 (yeast), HSMAD2, MAD2

Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, and MAD2, is a nucleus and cytoplasm protein that belongs to the MAD2 family. MAD2L1 is a component of the spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase-promoting complex by sequestering CDC2 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC2 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed-form and thereby promotes interaction with CDC2.

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MAD2L1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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Product Properties

参考文献

説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	Q13257
別名	MAD2 mitotic arrest deficient-like 1 (yeast), HSMAD2, MAD2
Construction	The Human MAD2L1 (Q13257) (Met 1-Asp 205) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	25.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, 20% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, and MAD2, is a nucleus and cytoplasm protein that belongs to the MAD2 family. MAD2L1 is a component of the spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase-promoting complex by sequestering CDC2 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC2 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed-form and thereby promotes interaction with CDC2.

参考文献

1. Luo X., et al., 2000, Nat. Struct. Biol. 7:224-229. 2. Sironi L., et al., 2002, EMBO J. 21:2496-2506. 3. Mapelli M., et al., 2007, Cell 131:730-743. 4. Ho C.-Y., et al., 2008, J. Cell. Biochem. 105:835-846. 5. Skinner J.J., et al., 2008, J. Cell Biol. 183:761-768.

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Keywords

MAD2L1 Protein, Human, Recombinant (His) MAD2 mitotic arrest deficient-like 1 (yeast) HSMAD2 MAD2 mitotic arrest deficient-like 1 MAD 2 HSMAD 2 MAD2 HSMAD-2 MAD-2 yeast recombinant recombinant-proteins proteins protein