Mer Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.7 kDa and the accession number is Q60805.
説明 | Mer Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.7 kDa and the accession number is Q60805. |
Species | Mouse |
Expression Host | Baculovirus Insect Cells |
Tag | His, GST |
Accession Number | Q60805 |
別名 | Eyk, Mer, MER proto-oncogene, tyrosine kinase, Nyk, nmf12 |
Construction | The Mouse MERTK (Q60805) (Glu573-Tyr867) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Protein Purity | > 96 % as determined by SDS-PAGE |
分子量 | 61.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in the membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in the testis, ovary, prostate, lung, and kidney, with lower expression in the spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and engulfment. MERTK plays also an important role in the inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces the production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Mer Protein, Mouse, Recombinant (His & GST) Eyk Mer MER proto-oncogene, tyrosine kinase Nyk nmf12 recombinant recombinant-proteins proteins protein