MERS-CoV Spike/RBD Protein fragment (aa 367-606, His)

MERS-CoV Spike/RBD Protein fragment (aa 367-606, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is K0BRG7.

MERS-CoV Spike/RBD Protein fragment (aa 367-606, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 134,000
200 μg	約5 days	￥ 228,500
500 μg	約5 days	￥ 461,500

バルクのお問い合わせ

生物学的情報	Testing in progress
説明	MERS-CoV Spike/RBD Protein fragment (aa 367-606, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is K0BRG7.

Species	MERS-CoV
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	K0BRG7
別名	cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, RBD Prote, ncov RBD Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov spike Protein, MERS-CoV
Construction	The MERS-CoV receptor binding domain (RBD) of spike protein fragment (AFS88936.1) (Glu367-Tyr606) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	27.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

参考文献

1. Xiao X,et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30. 2. Shen S,et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. 3. Du L,et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

MERS-CoV Spike/RBD Protein fragment (aa 367-606, His)

参考文献

Calculator

技術サポート

Keywords