MFGE8 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 42 kDa and the accession number is Q08431-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 在庫あり | ¥ 88,500 |
200 μg | 約5 days | ¥ 151,500 |
500 μg | 約5 days | ¥ 307,500 |
生物学的情報 | When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37℃. |
説明 | MFGE8 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 42 kDa and the accession number is Q08431-1. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | His |
Accession Number | Q08431-1 |
別名 | SPAG10, HMFG, milk fat globule-EGF factor 8 protein, hP47, BA46, SED1, MFGM, MFG-E8, HsT19888, EDIL1, OAcGD3S |
Construction | The Human MFGE8 isoform 1 (Q08431-1) (Met 1-Cys 387) was fused with a polyhistidine tag at the C-terminus. |
Protein Purity |
≥ 80 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
|
分子量 | 42 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. |
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Please read the User Guide of Recombinant Proteins for more specific information.
MFGE8 Protein, Human, Recombinant (His) BA 46 SPAG10 HMFG milk fat globule-EGF factor 8 protein SPAG-10 SED 1 hP47 BA46 SPAG 10 HsT 19888 SED1 MFGM MFG-E8 HsT-19888 HsT19888 BA-46 EDIL1 OAcGD3S SED-1 EDIL 1 EDIL-1 recombinant recombinant-proteins proteins protein