MICA Protein, Human, Recombinant (His), MICA*018

MICA Protein, Human, Recombinant (His), MICA*018 is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34 kDa and the accession number is AAH16929.1.

MICA Protein, Human, Recombinant (His), MICA*018

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 64,000
200 μg	約5 days	￥ 119,500
500 μg	約5 days	￥ 271,000

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized MICAh at 2 μg/mL (100 μL/well) can bind S4-Fc3L3-NKG2D , the EC50 of S4-Fc3L3-NKG2D is 120-300 ng/mL.
説明	MICA Protein, Human, Recombinant (His), MICA*018 is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 34 kDa and the accession number is AAH16929.1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	AAH16929.1
別名	PERB11.1, MGC21250, MIC-A, MGC111087, FLJ36918, FLJ60820, DAMA-345G11.2, MHC class I polypeptide-related sequence A
Construction	The Human MICA (AAH16929.1) extracellular domain (Met 1-Gln 308) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	34 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to the MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gamma-delta T cells, CD8+ alpha-beta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by the ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gamma-delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.

参考文献

1. Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106. 2. Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8. 3. Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4. 4. Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9. 5. Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.

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MICA Protein, Human, Recombinant (His), MICA*018

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