MIF Protein, Mouse, Recombinant

MIF Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 12.5 kDa and the accession number is P34884.

MIF Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 38,000
50 μg	約5 days	￥ 63,500
1 mg	約5 days	￥ 643,000

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説明	MIF Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 12.5 kDa and the accession number is P34884.

Species	Mouse
Expression Host	E. coli
Tag	Tag Free
Accession Number	P34884
別名	GIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), Glif
Construction	The Mouse MIF (P34884) precursor (Pro 2-Ala 115) was expressed, with an initial Met at the N-terminus.
Protein Purity	> 93 % as determined by SDS-PAGE
分子量	12.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 0.1 M NaCl, pH 8.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is a ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of the central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread, and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important role in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.

参考文献

1. Ohkawara T,et al.(2005) Pathophysiological roles of macrophage migration inhibitory factor in gastrointestinal, hepatic, and pancreatic disorders. J Gastroenterol. 40(2): 117-22. 2. Bach JP,et al.. (2009) The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer. 115(10): 2031-40. 3. Rendon BE,et al.. (2009) Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp Mol Pathol. 86(3): 180-5. 4. Grieb G,et al.(2010) Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News Perspect. 23(4): 257-64.

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MIF Protein, Mouse, Recombinant

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