VEGFC Protein, Mouse/Rat, Recombinant (aa 108-223, His)

VEGFC Protein, Mouse/Rat, Recombinant (aa 108-223, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P97953-1.

VEGFC Protein, Mouse/Rat, Recombinant (aa 108-223, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 68,000
100 μg	約5 days	￥ 116,500
200 μg	約5 days	￥ 198,500
500 μg	約5 days	￥ 402,000

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生物学的情報	1. Immobilized mouse/rat VEGFC-His at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc , The EC50 of mouse VEGFR3-Fc is 17.4-40.6 ng/mL. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.8 μg/mL.
説明	VEGFC Protein, Mouse/Rat, Recombinant (aa 108-223, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P97953-1.

Species	Mouse,Rat
Expression Host	HEK293 Cells
Tag	His
Accession Number	P97953-1
別名	vascular endothelial growth factor C
Construction	The Mouse / Rat VEGFC (NP_033532.1/O35757) (Ala108-Arg223) was expressed fused with a polyhistidine tag at the C-terminus. Mouse and Rat mature VEGFC sequences are identical.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	14.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.

参考文献

1. Joukov V, et al. (1997) Vascular endothelial growth factors VEGF-B and VEGF-C. J Cell Physiol. 173(2): 211-5. 2. Su JL, et al. (2007) The role of the VEGF-C/VEGFR-3 axis in cancer progression. Br J Cancer. 96(4): 541-5. 3. Anisimov A, et al. (2009) Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle. Circ Res. 104(11): 1302-12.

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VEGFC Protein, Mouse/Rat, Recombinant (aa 108-223, His)

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