NCAM1 Protein, Mouse, Recombinant (aa 20-711, His)

Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. NCAM1 Protein, Mouse, Recombinant (aa 20-711, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 77.75 kDa and the accession number is P13595-1.

NCAM1 Protein, Mouse, Recombinant (aa 20-711, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P13595-1
別名	NCAM-1, NCAM, CD56, MSK39, NCAM1, N-CAM-1
Construction	Leu20-Thr711
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	77.75 kDa (predicted). Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

参考文献

1. Guven A, et al. Development and validation of an immunoassay for quantification of NCAM-1 in human plasma. J Pharm Biomed Anal. 2021 Apr 15;197:11398doi: 10.1016/j.jpba.2021.11398Epub 2021 Feb 16. PMID: 33657526.

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NCAM1 Protein, Mouse, Recombinant (aa 20-711, His)

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