Nectin-2 Protein, Human, Recombinant

Nectin-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 36.2 kDa and the accession number is Q92692-2.

Nectin-2 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 77,000
200 μg	約5 days	￥ 131,500
500 μg	約5 days	￥ 266,500

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生物学的情報	1.Measured by its binding ability in a functional ELISA. Immobilized human CD112 at 10 μg/ml (100 μl/well) can bind human DNAM1-Fch with a linear range of 1.25-10 μg/ml. 2.Loaded Human PVRIG Protein, mFc Tag on AMC Biosensor, can bind Human Nectin-2 protein with an affinity constant of 0.680μM as determined in BLI assay (Routinely tested).
説明	Nectin-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 36.2 kDa and the accession number is Q92692-2.

Species	Human
Expression Host	HEK293
Tag	Tag Free
Accession Number	Q92692-2
別名	PRR2, Nectin-2, PVRR2, poliovirus receptor-related 2 (herpesvirus entry mediator B), HVEB, CD112
Construction	The mature form of recombinant human CD112 (NP_002847.1) extracellular domain (Met 1-Leu 360) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	36.2 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

参考文献

1. Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6. 2. Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.

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Nectin-2 Protein, Human, Recombinant

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