Neuropilin-1 Protein, Human, Recombinant (V179A, hFc)

Neuropilin-1 Protein, Human, Recombinant (V179A, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 96.5 kDa and the accession number is O14786-2.

Neuropilin-1 Protein, Human, Recombinant (V179A, hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 42,500
100 μg	約5 days	￥ 67,500
200 μg	約5 days	￥ 109,000

バルクのお問い合わせ

生物学的情報	Measured by its ability to bind with recombinant human VEGF165 in a functional ELISA.
説明	Neuropilin-1 Protein, Human, Recombinant (V179A, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 96.5 kDa and the accession number is O14786-2.

Species	Human
Expression Host	HEK293
Tag	hFc
Accession Number	O14786-2
別名	neuropilin 1, VEGF165R, CD304, NP1, Neuropilin-1, NRP, BDCA4
Construction	A DNA sequence encoding the human NRP1 isoform b (NP_001019799.1, with natural variant Val 179 Ala) (Met1-Lys644) was expressed with the Fc region of human IgG1 at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	96.5 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

参考文献

1. Nakamura F,et al.(2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69. 2. Romeo PH,et al.(2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54. 3. Klagsbrun M,et al.(2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48. 4. Staton CA,et al.(2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48. 5. Bagri A,et al.(2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

Neuropilin-1 Protein, Human, Recombinant (V179A, hFc)

参考文献

Calculator

技術サポート

Keywords