Neuropilin-1 Protein, Mouse, Recombinant (His)

Neuropilin-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 95.12 kDa and the accession number is P97333.

Neuropilin-1 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 41,500
100 μg	約5 days	￥ 72,500
200 μg	約5 days	￥ 128,500
500 μg	約5 days	￥ 271,000

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized VEGF165 can bind mNRP1h , the EC50 of mNRP1h is 30-120 ng/mL.
説明	Neuropilin-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 95.12 kDa and the accession number is P97333.

Species	Mouse
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P97333
別名	neuropilin 1, Npn1, Nrp, NP-1, NPN-1, C530029I03
Construction	Mouse NRP1 (NP_032763.2) (Met1-Pro856)
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	95.12 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

参考文献

1. Nakamura F,et al.(2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69. 2. Romeo PH,et al.(2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54. 3. Klagsbrun M,et al.(2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48. 4. Staton CA,et al.(2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48. 5. Bagri A,et al.(2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.

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Neuropilin-1 Protein, Mouse, Recombinant (His)

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